Selected Publications
Tapia-Rojo, R*; Mora, M*; Board, S.J.; Walker, J; Boujemaa-Paterski, R.; Medalia, O; Garcia-Manyes, S. «Enhanced statistical sampling reveals microscopic complexity in the talin mechanosensor folding energy landscape», Nature Physics (2023), 19 (1), 52-60. Link
Beedle A, Garcia-Manyes, S., «The role of single-protein elasticity in mechanobiology», Nature Reviews Materials (2023), 8, 10-24. Link
Mora, M; Stannard, A.; Garcia-Manyes, S. «The nanomechanics of individual proteins», Chemical Society Reviews (2020), 49, 6816-6832. Link
Infante, E*; Stannard, A*; Board, S.J.; Rico-Lastres, P.; Panagaki, F.; Beedle, A.E.M.; Sundar Rajan, V.; Rostkova, E.; Lezamiz, A.; Wang, Y.; Gulaidi, S.; Shanahan, C.; Roca-Cusachs, P.; Garcia-Manyes, S. «The mechanical stability of proteins regulates their translocation rate into the cell nucleus», Nature Physics (2019), 15, 973–981. Link
Beedle, A. E*., Mora, M*., Davis, C., Snijders, A., Stirnemann, G., Garcia-Manyes, S. «Forcing the reversibility of a mechanochemical reaction» Nature Communications (2018), 9, 3155. Link
Perales-Calvo, J., Giganti, D., Stirnemann, G., Garcia-Manyes, S. «The force-dependent mechanisms of DnaK-mediated mechanical folding» Science Advances (2018) 4, 2. Link
Garcia-Manyes, S., Beedle, A.E.M. «Steering chemical reactions with force» Nature Reviews Chemistry (2017) Link
Elosegui-Artola, A., Andreu, I., Beedle, A.E.M., Lezamiz, A., Uroz, M., Kosmalska, A., Oria, R., Kechagia, J., Rico-Lastres, P., Le Roux, A., Shanahan, C., Trepat, X., Navajas, D., Garcia-Manyes, S., Roca-Cusachs, P. «Force triggers YAP entry by mechanically regulating transport across nuclear pores» Cell (2017), 171, 1-14. Link
Beedle, A. E., Mora, M., Lynham, S., Stirnemann, G., Garcia-Manyes, S. «Tailoring protein nanomechanics with chemical reactivity» Nature Communications (2017), 8, 15658. Link PDF: ncomms15658
Beedle, A. E.; Lynham, S.; Garcia-Manyes, S. «Protein S-sulfenylation is a fleeting molecular switch that regulates non-enzymatic oxidative folding » Nature Communications (2016), 7, 12490. Link. PDF: ncomms12490
Beedle, A. E.; Lezamiz, A.; Sitrnemann, G.; Garcia-Manyes, S. « The mechanochemistry of copper reports on the directionality of unfolding in model cupredoxin proteins» Nature Communications (2015), 6, 7894. Link PDF: ncomms8894
Atilla-Gokcumen, G.; Muro, E.; Relat-Goberna, J.; Sasse, S.; Bedigian, A.; Coughlin, M.; Garcia-Manyes, S.; Eggert, U. «Dividing cells regulate their lipid composition and localization» Cell (2014), 3, 428-439. PDF: Cell2014
Popa, I; Kosuri, P.; Alegre-Cebollada, J.; Garcia-Manyes, S.; Fernandez, J.M. «Force dependency of biochemical reactions measured by single molecule force-clamp spectroscopy» Nature Protocols (2013), 8, 1261-1276. pdf: nprot.2013-Popa
All Publications (by year)
2023
Bernabe-Rubio, M; Ali, S; Bhosale, P; Goss, G; Mobasseri AS; Tapia-Rojo, R; Zhu, T; Battilocchi, M; Tomas, I; Ganier, C; Garcia-Manyes S; Watt, F «Mechanical factors induce Myc-dependent dedifferentiation of adult epidermal cells via direct reversal of the terminal differentiation trajectory» Nature Cell Biology (2023), 25, 1426–1438.
Martin-Baniandres, P; Lan, W; Board, S; Romero-Ruiz, M; Garcia-Manyes, S; Qing, S.; Bayley, H. «Enzyme-less nanopore detection of post-translational modifications within long polypeptides», Nature Nanotechnology (2023), https://doi.org/10.1038/s41565-023-01462-8.
Franz, F; Tapia-Rojo, R; Winograd-Katz, S; Boujemaa-Paterski, R; Li, W; Unger, T; Albeck, S; Aponte-Santamaria, C; Garcia-Manyes, S; Medalia, O; Geiger, B; Grater, F. «Allosteric activation of vinculin by talin», Nature Communications (2023), 14:4311.
Tapia-Rojo, R*; Mora, M*; Board, S.J.; Walker, J; Boujemaa-Paterski, R.; Medalia, O; Garcia-Manyes, S. «Enhanced statistical sampling reveals microscopic complexity in the talin mechanosensor folding energy landscape», Nature Physics (2023), 19 (1), 52-60. Link
Beedle A, Garcia-Manyes, S., «The role of single-protein elasticity in mechanobiology», Nature Reviews Materials (2023), 8, 10-24. Link
2022
Andreu, I; Granero-Moya, I; Garcia-Manyes S.; Roca-Cusachs, P. «Understanding the role of mechanics in nucleocytoplasmic transport», APL Bioengineering (2022); 6, 020901. Link
Mora M, Board S, Languin-Cattoën, O, Masino L, Stirnemann G, Garcia-Manyes S. «A single molecule strategy to capture non-native intramolecular and intermolecular disulfide bonds», Nano Letters (2022); 22, 10, 3922–3930. Link.
2021
Wallis SS, Ventimiglia, LN, Otigbah E, Infante E, Cuesta-Geijo MA, Kidiyoor GR, Carbajal MA, Fleck RA, Foiani M, Garcia-Manyes S, Martin-Serrano J, Agromayor M «The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair» Developmental Cell (2021), 56, 23, 3192-3202. Link.
Stannard A, Mora M, Beedle AEM, Castro-Lopez M, Board S, Garcia-Manyes S. «Molecular fluctuations as a ruler of force-induced protein conformations», Nano Letters (2021); 21, 7, 2953–2961. Link.
2020
Mora M; Garcia-Manyes S. «Protein nanomechanics: The power of stretching» Europhysics News (2020), 51, 5, 24-27.
Mora, M; Stannard, A.; Garcia-Manyes, S. «The nanomechanics of individual proteins», Chemical Society Reviews (2020), 49, 6816-6832. Link
2019
Infante, E; Stannard, A; Board, S.J.; Rico-Lastres, P.; Panagaki, F.; Beedle, A.E.M.; Sundar Rajan, V.; Rostkova, E.; Lezamiz, A.; Wang, Y.; Gulaidi, S.; Shanahan, C.; Roca-Cusachs, P.; Garcia-Manyes, S. «The mechanical stability of proteins regulates their translocation rate into the cell nucleus», Nature Physics (2019), 15, 973–981. Link
Coker, H.L.E, Cheetham, M.R., Kattnig, D.R., Wang, Y.J., Garcia-Manyes, S., Wallace, M.I. «Controlling anomalpus diffusion in lipid membranes» Biophysical Journal (2019) 116, 6, 1085. Link
Mobasseri, S.A., Zijl, S., Salameti, V., Walko, G., Stannard, A., Garcia-Manyes, S. Watt, F.M. «Patterning of human epidermal stem cells on ondulating elastomer substrates reflects differences in cell stiffness» Acta Biomaterialia (2019) 87, 256. Link
2018
Beedle, A. E*., Mora, M*., Davis, C., Snijders, A., Stirnemann, G., Garcia-Manyes, S. «Forcing the reversibility of a mechanochemical reaction» Nature Communications (2018), 9, 3155. Link
Wang, Y*., Rico-Lastres, P*., Lezamiz, A*., Mora, M., Solsona, C., Stirnemann, G., Garcia-Manyes, S. «DNA binding induces a nanomechanical switch in the RRM1 domain of TDP-43» The Journal of Physical Chemistry Letters (2018) 9, 14. Link
Perales-Calvo, J., Giganti, D., Stirnemann, G., Garcia-Manyes, S. «The force-dependent mechanisms of DnaK-mediated mechanical folding» Science Advances (2018) 4, 2. Link
2017
Garcia-Manyes, S., Beedle, A.E.M. «Steering chemical reactions with force» Nature Reviews Chemistry (2017) Link
Elosegui-Artola, A., Andreu, I., Beedle, A.E.M., Lezamiz, A., Uroz, M., Kosmalska, A., Oria, R., Kechagia, J., Rico-Lastres, P., Le Roux, A., Shanahan, C., Trepat, X., Navajas, D., Garcia-Manyes, S., Roca-Cusachs, P. «Force triggers YAP entry by mechanically regulating transport across nuclear pores» Cell (2017), 171, 1-14. Link
Beedle, A. E., Mora, M., Lynham, S., Stirnemann, G., Garcia-Manyes, S. «Tailoring protein nanomechanics with chemical reactivity» Nature Communications (2017), 8, 15658. Link PDF: ncomms15658
Relat-Goberna, J., Beedle, A.E.M., Garcia-Manyes, S. «The nanomechanics of lipid multibilayer stacks exhibits complex dynamics» Small (2017), 13, 1700147. Link PDF: Relat-Goberna_et_al-2017-Small
Pernigo, S., Fukuzawa, A., Beedle, A.E.M., Holt, M., Round, A., Pandini, A., Garcia-Manyes, S., Gautel, M., Steiner, R.A. «Binding of Myomesin to Obscurin-Like-1 at the Muscle M-Band Provides a Strategy for Isoform-Specific Mechanical Protection» Structure (2017), 25, 107-120. PDF: 2017Structure
2016
Beedle, A. E.; Lynham, S.; Garcia-Manyes, S. «Protein S-sulfenylation is a fleeting molecular switch that regulates non-enzymatic oxidative folding » Nature Communications (2016), 7, 12490. Link. PDF: ncomms12490
2015
Garcia-Manyes, S.; Giganti,D.; Badilla, C. L.; Lezamiz, A.; Perales-Calvo, J.; Beedle, A. E.; Fernandez, J. M. «Single molecule force spectroscopy predicts a misfolded domain-swapped conformation in the human γD- crystallin» The Journal of Biological Chemistry (2015) DOI: jbc.M115.673871. Link PDF: J. Biol. Chem.-2015
Beedle, A. E.; Williams, A.; Relat-Goberna, J.; Garcia-Manyes, S. «Mechanobiology – chemical origin of membrane mechanical resistance and force-dependent signaling» Current Opinion in Chemical Biology (2015), 29, 87-93. Link PDF: 2015currentopinion
Perales-Calvo, J.; Lezamiz, A.; Garcia-Manyes, S. «The mechanochemistry of a structural zinc finger» The Journal of Physical Chemistry Letters (2015), 6, 3335-3340. Link PDF: PeralesCalvo-PhysChemLett
ACS Editors Choice
Beedle, A. E.; Lezamiz, A.; Sitrnemann, G.; Garcia-Manyes, S. « The mechanochemistry of copper reports on the directionality of unfolding in model cupredoxin proteins» Nature Communications (2015), 6, 7894. Link PDF: ncomms8894
Relat-Goberna, J.; Garcia-Manyes, S. «Direct observation of the dynamics of self-assembly of individual solvation layers in molecularly confined liquids» Phys. Rev. Lett (2015), 114 (25) 258303. Link PDF: PhysRevLett.114.258303
2014
Atilla-Gokcumen, G.; Muro, E.; Relat-Goberna, J.; Sasse, S.; Bedigian, A.; Coughlin, M.; Garcia-Manyes, S.; Eggert, U. «Dividing cells regulate their lipid composition and localization» Cell (2014), 3, 428-439. PDF: Cell2014
2011-2013
Popa, I; Kosuri, P.; Alegre-Cebollada, J.; Garcia-Manyes, S.; Fernandez, J.M. «Force dependency of biochemical reactions measured by single molecule force-clamp spectroscopy» Nature Protocols (2013), 8, 1261-1276. pdf: nprot.2013-Popa
Berkovich, R.; Hermans, R.I.; Popa, I.; Stirnemann, G.; Garcia-Manyes, S.; Berne, B.J.; Fernandez, J.M; «Rate limit of protein elastic response is tether dependent» Proc. Natl. Acad. Sci. USA, (2012), 109 (36) 14416-14421. pdf: PNAS-2012-Berkovich
Garcia-Manyes, S*; Badilla, C.L.; Alegre-Cebollada, J.; Javadi, Y.; Fernandez, J.M. «Spontaneous dimerization of the titin Z1-Z2 domains induces a strong nano-mechanical anchorin», Journal of Biological Chemistry (2012); 287 (24) 20240-20247. pdf: JBC-2012-Garcia-Manyes
Popa, I; Fernández, J.M.; Garcia-Manyes, S.*; «Direct quantification of the attempt frequency determining the mechanical unfolding of ubiquitin protein», Journal of Biological Chemistry (2011); 286 (36), 31072-31079. pdf: JBC-2012-Popa
Garcia-Manyes, S*; Kuo, TL.; Fernández, J.M., «Contrasting the individual reactive pathways in protein unfolding and disulfide bond reduction observed within a single protein», Journal of the American Chemical Society (2011), 133 (9), 3104-3113. pdf: JACS-2011-Garcia-Manyes
Perez-Jimenez, R.; Inglés-Prieto, A.; Zhao, Z.M.; Sanchez-Romero, I.; Alegre-Cebollada J.; Kosuri, P.; Garcia-Manyes, S.; Holmgren, A.; Kappock, T.J.; Tanokura, M.; Sanchez-Ruiz J.M.; Gaucher, E.A.; Fernandez, J.M., «Paleoenzymology at the single-molecule level: probing the chemistry of resurrected enzymes», Nature Structural & Molecular Biology (2011), 18, 592-596. pdf: NSMB-2011-Perez-Jimenez
2006-2011
Garcia-Manyes, S*; «Caught in the act», Nature Chemistry, (2010), 2, 905-906. pdf:
Garcia-Manyes, S.*; Redondo-Morata, L.; Oncins, G.; Sanz, F.; «Nanomechanics of lipid bilayers: heads or tails?», Journal of the American Chemical Society (2010), 132 (7) 12874-12886. pdf: JACS-2010-Garcia-Manyes
Berkowitz, R.; Garcia-Manyes, S.; Klafter, J.; Urback, M.; Fernández, J.M.; «Hopping around an entropic barrier created by force», Biochemical and Biophysical Research Communications (2010), 403, 133-137. pdf: BBRC-2010-Garcia-Manyes
Kuo, TL.; Garcia-Manyes, S.; Li, J.; Barel, I.; Lu, H.; Berne, B.J.; Urbakh, M.; Klafter, J.; Fernández, J.M., «Probing disorder in Arrhenius with single molecule force spectroscopy», Proc. Natl. Acad. Sci. USA, (2010), 107 (25) 11336-11340. pdf: PNAS-2010-Kuo
Berkowitz, R.; Garcia-Manyes, S.; Klafter, J.; Urback, M.; Fernández, J.M.; «Collapse dynamics of single proteins extended by force», Biophysical Journal, 2010, 98, 2692-2701. pdf: BJ-2010-Garcia-Manyes
Garcia-Manyes, S.*; Sanz, F.; «Nanomechanics of lipid bilayers by force spectroscopy with AFM: a perspective», Biochimica et Biophysica Acta: Biomembranes, (invited review), (2010), 1798, 741-749. pdf: BBA-2010-Garcia-Manyes
Garcia-Manyes, S.*; Dougan, L.; Fernandez, J.M.; «Osmolyte induced separation of the mechanical folding phases of ubiquitin», Proc. Natl. Acad. Sci. USA, (2009), 106 (26)10540-10545. pdf: PNAS-2009-Garcia-Manyes2
Garcia-Manyes, S.*; Dougan, L.; Badilla, C. L.; Brujic, J; Fernandez, J.M.; «Direct observation of an ensemble of stable collapsed states in the mechanical folding of ubiquitin», Proc. Natl. Acad. Sci. USA, (2009), 106 (26),10534-10539. pdf: PNAS-2009-Garcia-Manyes1
Garcia-Manyes, S.*; Liang, J.; Szoszkiewicz, R.; Kuo, T.; Fernandez, J.M.; «Force-activated reactivity switch in a bimolecular chemical reaction», Nature Chemistry, (2009), 1, 236-242. Featured in the News and Views of the same issue, Frank, I and Hofbauer, F., Nature Chemistry (2009) 1, 180-181. pdf: NChem-2009-Garcia-Manyes
Fernandez, J.M., Garcia-Manyes, S.; Dougan, L. «Force-clamp spectroscopy of single proteins», in Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium, Edited by Astrid Gräslund, Rudolf Rigler and Jerker Widengren (Springer Series in Chemical Physics), 2009.
Liu, R.; Garcia-Manyes*, S.; Sarkar, S.; Badilla, C.L.; Fernandez, J.M.; «Mechanical characterization of Protein L in the low-force regime by electromagnetic tweezers/evanescent nanometry», Biophysical Journal, (2009), 96, 3810-3821. pdf: BJ-2009-Garcia-Manyes
Grüntzer, A.; Garcia-Manyes, S.; Badilla, C.L., Fernandez, J.M.; Linke, W.A.; «Modulation of titin-based stiffness by dislufide bonding in the cardiac titin N2B unique sequence», Biophysical Journal, (2009), 97, 825-834. pdf: BJ-2009-Grutzner
Garcia-Manyes, S.; Brujic, J.; Badilla, C. L.; Fernandez, J. M.; «Force-clamp spectroscopy of single protein monomers reveal the individual unfolding and folding pathways of I27 and ubiquitin», Biophysical Journal (2007), 93, 2436-2446. pdf: BJ-2007-Garcia-Manyes
Brujic, J.; Hermans, R.I.Z.; Garcia-Manyes, S.; Walther, K.A.; Fernandez, J. M.; «Dwell-time distribution analysis of polyprotein unfolding using ‘Force-Clamp’ Spectroscopy», Biophysical Journal (2007), 92, 2896-2903. pdf: BJ-2007-Brujic
Perez-Jimenez, R.; Garcia-Manyes, S., Ainavarapu, S.R.K, Fernandez, J.M., «Mechanical Unfolding Pathways of the Enhanced Yellow Fluorescent Protein Revealed by Single Molecule Force Spectroscopy», Journal of Biological Chemistry (2006), 281 (52), 40010-40014.
2002-2006
Garcia-Manyes, S.; Bucior, I.; Ros, R.; Anselmetti, D:; Sanz, F.; Burger, M. M.; Fernandez-Busquets, X., «Cell adhesion in the oldest extant Metazoans is based on strong interactions of allotypic glycans that can discriminate self from non-self» Journal of Biological Chemistry (2006), 281 (9), 5992-5999.
Garcia-Manyes, S.; Gorostiza, P.; Sanz, F., «Titration force microscopy on supported lipid bilayers», Analytical Chemistry (2006), 78, 61-70.
Garcia-Manyes, S.; Oncins, G.; Sanz, F., «Effect of temperature on the nanomechanics of lipid bilayers studied by Force Spectroscopy», Biophysical Journal (2005), 89 (6), 4261-4274.
Garcia-Manyes, S.; Oncins, G.; Sanz, F., «Effect of ion-binding and chemical phospholipid structure on the nanomechanics of lipid bilayers studied by Force Spectroscopy», Biophysical Journal (2005), 89 (3), 1812-1826.
Morant, C.; Garcia-Manyes, S.; Sanz, F.; Sanz, J. M.; Elizalde, E., «Nanotribological properties of CN/TiCN/TiN/Si multilayer as determined by AFM», Nanotechnology (2005), 16, 211-217.
Oncins, G.; Garcia-Manyes, S.; Sanz, F., «Study of Frictional Properties of 1,2-Dimyristoyl-sn-glycero-3-phosphocholine Bilayers in Liquid Environment», Langmuir (2005), 21, 7373-7379.
Garcia-Manyes, S., Güell, A. G.; Gorostiza, P.; Sanz, F., «Nanotribological properties of CN/TiCN/TiN/Si multilayer as determined by AFM», Journal of Chemical Physics (2005), 123, 114711. (Also selected by the Virtual Journal of Nanoscale Science & Technology (2005), 12, 14).
Garcia-Manyes, S.; Verdaguer, A.; Gorostiza, P.; Sanz, F., «Alkali Halide Nanocrystal Growth and Etching Studied by AFM and Modeled MD Simulation», Journal of Chemical Physics (2004), 120, 6, 2963-2971.
Fraxedas, J.; Garcia-Manyes, S.; Gorostiza, P.; Sanz, F., «Nanoindentation: towards the sensing of atomic interactions», Proc. Natl. Acad. Sci. USA (2002), 99, 5228-5232.
2002
Garcia-Manyes, S.; Jiménez, G.; Padró, T.; Rubio, R.; Rauret, G., «Arsenic speciation in contaminated soils», Talanta (2002), 58, 97-109.